antibodies against fitc cd45  (Elabscience Biotechnology)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A novel agarose-free, standardized generation and versatile ECM characterization of decellularized scaffolds from normal and fibrotic human lung tissue

doi: 10.3389/fbioe.2026.1772891

Figure Lengend Snippet: Cultivation of highly pure human primary fibroblasts from fibrotic end-stage lungs. (A–F) Fibroblast culture in early and late stages. (A) High-power magnification of a lung tissue cell isolate 24 h after tissue dissociation and seeding. Various adherent (asterisks) and non-adherent (arrows) cell types are visible. (B) High-power magnification of a lung tissue cell isolate 24 h after tissue dissociation and after one wash/medium change. Non-adherent cells (arrows) have been reduced significantly. Fibroblast-like (elongated) phenotypes (spiky arrows) are visible. (C, D) High- (C) and low-power (D) magnification of the cell culture after 7 days. Activated fibroblasts (dagger) that are increased in size start to form cluster. (E) Low-power magnification of the cell culture after 14 days. Fibroblasts (dagger) start to overgrow other cell types (asterisks). (F) Overview of a 21-day-old culture that has been split once. Fibroblasts (dagger) cover approximately 99% of the surface of the culture plate, other cell types are not visible. Scale bars: 100 μm Original magnifications: ×200 (A–C) ; ×100 (D, E) ; ×40 (F) . (G) Gating strategy to validate the purity of fibroblasts in passage 7 before co-culture. Top section: Isotype control: Exemplary sample of primary human fibroblasts incubated with IgG-FITC, IgG-APC and IgG-VioBlue at passage 7. Bottom section: Exemplary sample of primary human fibroblasts stained with anti-CD90 (conjugate: FITC), anti-CD31 (conjugate: APC) and anti-CD45 (conjugate: VioBlue) at passage 7. APC, Allophycocyanin; FITC, Fluorescein isothiocyanate; FSC-A, Forward scatter area; FSC-H, Forward scatter height; IgG, Immunoglobulin G; SSC-A, Side scatter area.

Article Snippet: Fibroblasts (500,000 cells; passage 7) were resuspended in 100 μL MACS buffer (0.5% (w/v) BSA, 2 mM EDTA) containing fluorophore-conjugated antibodies against CD90 (FITC Anti-human CD90 (Thy-1), clone REA897 Miltenyi Biotec, Cat. No.: 130-114-859), CD31 (APC Anti-human CD31, clone REA1028, Miltenyi Biotec, Cat. No.: 130-117-226), CD45 (VioBlue Anti-human CD45, clone REA747, Miltenyi Biotec, Cat. No.: 130-110-637) each 1:50 dilution or isotype controls (all from Miltenyi Biotec: Isotype FITC recombinant human IgG1, clone REA293, Cat. No.: 130-118-354; Isotype VioBlue recombinant human IgG1, REA293, Cat. No.: 130-117-362; Isotype APC recombinant human IgG1, REA293, Cat. No.: 130-120-709).

Techniques: Cell Culture, Co-Culture Assay, Control, Incubation, Staining

Journal: Cell Transplantation

Article Title: Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice

doi: 10.1177/09636897251376125

Figure Lengend Snippet: Characteristics of adipose-derived stem cells. (a) Positive expression for CD90 and CD105 and negative expression for CD19 and CD34. (b), (c) Differentiation of ADSCs into adipocytes and osteoblasts. After adipogenic differentiation, newly differentiated adipocytes had lipid droplets identified by Oil Red O staining. Osteogenic differentiation was confirmed by Alizarin Red S staining. ADSCs: adipose-derived stem cells; CD: cluster of differentiation.

Article Snippet: Passage 3 cells were stained with antibodies against CD90 (E-AB-F1283C) (Elabscience, Wuhan, China), CD105 (E-AB-F1233UE) (Elabscience, Wuhan, China), CD19 (E-AB-F1004E) (Elabscience, Wuhan, China), and CD34 (E-AB-F1284D) (Elabscience, Wuhan, China).

Techniques: Derivative Assay, Expressing, Staining